Transcription profiling of mouse kidneys from Glis2-deficient animals

Glis2 is a member of the Gli-similar (Glis) subfamily of Kruppel-like zinc finger transcription factors. To obtain insights into the physiological functions of Glis2, mice deficient in Glis2 expression were generated. These mutant mice develop progressive renal disease associated with fibrosis and inflammation. Gene expression profiles were examined to identify changes in gene expression at early stages of progressive renal disease in Glis2 mutant mice. Experiment Overall Design: To analyze changes in gene expression, microarray analysis was performed using RNA from kidneys of PND25 and PND60 wild type and Glis2-mutant mice. Experiment Overall Design: Gene expression profiles were compared between kidneys of PND25 wt and Glis2 mutant mice and between PND60 wt and Glis2 mutants. RNA from three kidneys were pooled. RNA from two independent experiments was used and each microarray analysis was performed in duplicate.Glis2是Kruppel样锌指转录因子家族中Gli相似（Glis）亚家族的成员。为深入探究Glis2的生理功能，研究人员构建了Glis2表达缺陷型小鼠。该类突变小鼠会罹患进行性肾病，伴随纤维化与炎症反应。为鉴定Glis2突变小鼠在进行性肾病早期阶段的基因表达变化，研究人员对基因表达谱进行了检测。 实验整体设计：为分析基因表达变化，研究人员采用出生后第25天（PND25）与出生后第60天（PND60）的野生型（wild type，WT）及Glis2突变型小鼠的肾脏RNA开展微阵列分析。 实验整体设计：分别对比出生后第25天野生型（wt）与Glis2突变型小鼠的肾脏基因表达谱，以及出生后第60天野生型（wt）与突变型小鼠的肾脏基因表达谱。实验中将3只小鼠的肾脏RNA混合后制备样本；共使用两次独立实验提取的RNA，且每组微阵列分析均进行两次重复实验。