Plasma proteome profiling of Osteoporosis Sarcopenia (OsteoSarc) cohort

Sarcopenia is an age-related condition characterized by progressive decline in muscle mass, strength, and physical performance, contributing to frailty and adverse health outcomes. Despite increasing interest in molecular biomarkers, longitudinal data with external validation are limited. This study utilized high-throughput proteomic analysis to identify and validate biomarkers associated with progression of sarcopenia in two independent prospective cohorts. The OsteoSarc cohort (n=93) was followed up for two years. Plasma proteomic profiling was conducted using data-independent acquisition (DIA) mass spectrometry. Statistical analyses included multivariable regression, principal component analysis, and pathway enrichment.