Single cell RNA-seq of 444 epithelial cells from the mammary glands of pubescent and adult mice

Mammary glands were collected from 8 pubescent (4.7-4.9 week-old) female mice and 8 adult (10 week old) female mice. Freshly sorted epithelial cells were submitted to a Fluidigm C1 System machine for single cell capture and cDNA synthesis. Cells were visualized under the microscope to ensure integrity of the captured single cells prior to cDNA preparation. Libraries were prepared using the Nextera XT kit and sequencing was carried out on an Illumina NextSeq 500 to achieve paired-end 75 bp reads. RNA-seq profiling was completed for 221 cells from pubescent mice and 223 cells from adult mice.本数据集的样本取自8只青春期（4.7-4.9周龄）雌性小鼠与8只成年（10周龄）雌性小鼠的乳腺组织。新鲜分选得到的上皮细胞经Fluidigm C1系统（Fluidigm C1 System）完成单细胞捕获与互补脱氧核糖核酸（complementary DNA，cDNA）合成。在进行cDNA制备前，通过显微镜观察以确保捕获单细胞的完整性。测序文库采用Nextera XT试剂盒（Nextera XT kit）构建，并依托Illumina NextSeq 500测序平台开展双端75碱基读长的测序。最终完成了青春期小鼠来源的221个细胞与成年小鼠来源的223个细胞的RNA测序（RNA-seq）转录组谱分析。