Soybean Compact Mutant

Genome resequencing for mutation discovery in soybean short internode mutant. Whole-genome sequencing of the mutant revealed an 8.7kb deletion in the promoter of the Glyma.17g145200 gene, which encodes a member of the class III gibberellin 2-oxidases. The mutation has a dominant effect, likely via increased expression of the GA 2-oxidase transcript observed in green tissue as a result of the deletion in the promoter of Glyma.17g145200. Levels of GA precursors are altered in the Compact mutant, supporting a role in gibberellin metabolism, and the mutant phenotype can be rescued with exogenous GA3.

对大豆短茎突变体的基因组进行重测序以发现突变。突变体的全基因组测序揭示了Glyma.17g145200基因启动子区域存在8.7千碱基对的缺失，该基因编码III类赤霉素2-氧化酶的一个成员。该突变具有显性效应，可能通过Glyma.17g145200启动子缺失导致的GA 2-氧化酶转录本在绿色组织中的表达增加来实现。Compact突变体中GA前体的水平发生改变，这支持其在赤霉素代谢中的作用，且突变体的表型可通过外源GA3的添加得到挽救。