MiRNA Expression Profiling and Clinical Implications in Prostate Cancer Across Various Stages

This study aims to screen and identify microRNA (miRNA) expression profiles across different stages of prostate cancer (PCa) and benign prostatic hyperplasia (BPH) using high-throughput sequencing. The study seeks to determine whether specific miRNAs show consistent differential expression across various stages of PCa, with the goal of identifying potential biomarkers relevant to disease progression. A total of 12 specimens from PCa and BPH were collected from September 2021 to June 2022 at the Second Affiliated Hospital of Nanchang University, China (including 3 specimens from early localized tumors, local invasion, and distant metastasis tumors, and 3 specimens from BPH). miRNA expression profiles were screened using high-throughput sequencing, and differentially expressed miRNAs were identified using bioinformatics analysis. Further analysis, including target miRNA site analysis, GO enrichment analysis, and KEGG enrichment analysis of miRNA-derived genes, was performed. The expression of hsa-miR-6715b-3p in PCa tissues was verified using qRT-PCR.