Gene expression profiling at early organogenesis in lung and thyroid

The thyroid and lungs originate as neighboring bud shaped outgrowths from the midline of the embryonic foregut. When and how organ specific programs regulate development into structures of distinct shapes, positions and functions is not known. To characterize, at least in part, the genetic basis of these events, we have employed laser capture microdissection and microarray analysis to define gene expression in the mouse thyroid and lung primordia at E10.5. By comparing the transcriptome of each bud to that of the whole embryo as well as to each other, we broadly describe the genes that are preferentially expressed in each developing organ as well as those with an enriched expression common to both.甲状腺与肺均起源于胚胎前肠中线处的相邻芽状突起。目前尚不清楚器官特异性发育程序是如何、在何时调控二者发育为形态、位置与功能各异的器官结构的。为至少部分阐明上述过程的遗传基础，我们采用激光捕获显微切割（Laser Capture Microdissection）与微阵列（Microarray）分析技术，对胚胎发育第10.5天（E10.5）的小鼠甲状腺及肺原基的基因表达谱进行了解析。通过将这两个原基的转录组与全胚胎转录组以及二者彼此的转录组进行比对，我们系统描述了在各自发育器官中优先表达的基因，以及二者共有的高表达基因。