T. cruzi strain panel amastigote doubling time (DT), average ±SD, calculated using 0-72h growth curve data [see Methods] for at least 3 biological replicates.

This value was used to calculate the number of divisions in 72 h. Number of divisions in 120 h were calculated from DT determined using number of amastigotes at 0 and 120 h. Cycling time defines the time in days between the start of Vero cell infection with T. cruzi tissue culture derived trypomastigotes and the emergence of trypomastigote parasites. Level of parasite infection (Day 0) for each strain represented as percent infected Vero cells (average± SD).