Osteopontin expression identifies a subset of recruited macrophages distinct from Kupffer cells in the fatty liver. [Single cell RNA Seq]

CITE-seq analysis on total CD45+ cells isolated from the livers of mice fed a standard diet (SD) or western diet (WD; fat, cholesterol and sugar) for 12, 24 and 36 weeks. We also performed single cell RNA sequencing analysis of the CD45- cells isolated from the livers of the mice fed the SD or WD for 24 and 36 weeks. Single cell RNA Seq = Liver CD45+ and CD45- cells derived from mice fed a standard diet (SD) or western diet (WD; fat, cholesterol and sugar) for 12, 24 and 36 weeks. Bulk RNA Seq = Kupffer cells, moKCs and Clec4f- macrophages derived from mice fed a standard diet (SD) or western diet (WD; fat, cholesterol and sugar) for 12 and 24 weeks.本数据集针对喂食标准饮食（SD）或西式饮食（WD，含脂肪、胆固醇与蔗糖）12、24及36周的小鼠肝脏分离的总CD45阳性细胞开展了CITE-seq分析。同时，本研究还对喂食SD或WD 24、36周的小鼠肝脏分离的CD45阴性细胞进行了单细胞RNA测序分析。单细胞RNA测序（scRNA-seq）样本：来自喂食标准饮食（SD）或西式饮食（WD，含脂肪、胆固醇与蔗糖）12、24及36周的小鼠肝脏分离的CD45阳性与CD45阴性细胞。批量RNA测序（Bulk RNA Seq）样本：来自喂食标准饮食（SD）或西式饮食（WD，含脂肪、胆固醇与蔗糖）12及24周的小鼠肝脏分离的库普弗细胞（Kupffer cells）、单核细胞源性库普弗细胞（moKCs）以及Clec4f阴性巨噬细胞。