Norman et al., 2019 (Science) labeled Perturb-seq data

AnnData object containing single-cell gene expression levels of from a large-scale Perturb-seq CRISPR experiment from "Exploring genetic interaction manifolds constructed from rich single-cell phenotypes". Cells were labeled according to perturbation categories provided by the original authors. Data preprocessed as decribed in "Isolating salient variations of interest in single-cell data with contrastiveVI" (Nature Methods 2023).

AnnData 对象收录了来自论文"Exploring genetic interaction manifolds constructed from rich single-cell phenotypes"（探索基于丰富单细胞表型的遗传相互作用流形）中的大规模扰动测序（Perturb-seq）CRISPR实验的单细胞基因表达水平。所有细胞均依据原作者提供的扰动类别完成标注。数据预处理流程严格遵循论文"Isolating salient variations of interest in single-cell data with contrastiveVI"（2023年发表于《自然-方法》（Nature Methods））中的描述。

搜集汇总

背景与挑战

背景概述

该数据集包含基于CRISPR的Perturb-seq大规模实验的单细胞基因表达数据，经过预处理并标注了原始作者提供的扰动类别，存储格式为AnnData。数据集采用CC BY 4.0许可协议，适用于基因表达相关研究。