Stromal-epithelial interaction via soluble factors in prostate carcinoma cell lines

Cell-cell communication is an important process in healthy tissue but also gains enhanced attention regarding pathological tissue. To date, the tumor microenvironment is gradually brought into focus when studying tumorigenesis. In the prostate gland, stromal and epithelial cells greatly interact to maintain homeostasis or tissue integrity. This study focuses on an indirect communication via soluble factors. For this approach, the prostate carcinoma cell line LNCaP and the stromal primary cells p21 were co-cultured without direct contact and RNA was isolated at defined time points (one, three and seven days). RNA sequencing finally revealed a time-depending differential expression profile. Comparative gene expression profiling analysis of RNA-seq data for co-cultured LNCaP and p21 cells interacting via soluble factors for one, three and seven days. Co-cultures with the same cell line were used as controls.