Statistical parameters of the FP assay.
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The performance of the assay was determined during initial optimization and after the HTS assay. Pc is the mean FP signal of PBP 2-bound tracer control, Nc is the mean FP signal of free Bocillin-FL, and Dc is the FP signal in the presence of 100 µm penicillin G. S/N (signal-to-noise) and the Z factors are as defined in Material and Methods. (n = number of measurements). See also Fig. S1).
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2015-12-02
