The genetic differentiation of the northern and southern Izu lineages of the Japanese newt Cynops pyrrhogaster was investigated through their single nucleotide polymorphisms variations by multiplexed ISSR genotyping by sequencing (MIG-seq).

To obtain de novo genome-wide SNP data for Cynops pyrrhogaster, multiplexed inter-simple sequence repeat (ISSR) genotyping by sequencing (MIG-seq) analysis (Suyama & Matsuki, 2015) were conducted. MIG-seq method is one of the PCR-based reduced-representation sequencings that has been conducted in various species (e.g., Matsui et al., 2019; Sato et al., 2021).