Senescence-Induced Immune Remodeling Facilitates Metastatic Adrenal Cancer in a Sex-Dimorphic Manner [scRNA-seq]

Using single-cell RNA-sequencing (scRNA-seq), we characterize diverse immune cell infiltrates that remodel the tissue microenvironment following senescence activation in female Znrf3 cKO mice. We performed scRNA-seq to provide an unbiased profile of the immune microenvironment following senescence activation. We specifically focused on the initial switch from hyperplasia (6-weeks) to regression (9-weeks) where we expected to identify the most proximal senescence-driven immune response. Adrenal glands were isolated from female SF1-Cre+;Znrf3-Fx/Fx mice at 6-weeks (n=2 adrenals) or 9-weeks (n=3 adrenals).