Test methods applied for detection of antibodies against M. leprae-specific antigens.

Overview of the details of the methods used in the studies (n = 30) reporting serology data on M. leprae-specific antibodies in children. For each study, the reference number, author, year of publication, country, sample, test target, nature of the test target, antibody isotype, assay, readout, dilution, cut-off and cut-off explanation are listed. Ab: antibody; CP: contact population from a leprosy colony; ELISA: enzyme-linked immunosorbent assay; F: females; GPAT: gelatin particle agglutination test; HER: children from reported high-endemic region; IgA: immunoglobulin A; IgG: immunoglobulin G; IgM: immunoglobulin M; LER: children from reported low-endemic region; LID-1: leprosy IDRI diagnostic-1; M: males; (N)D-O-BSA: (natural) disaccharide-octyl bovine serum albumin; ND-O-HSA: natural disaccharide-octyl human serum albumin; ND-O-LID: semi-synthetic disaccharide attached to the octyl radical, which mimics PGL-I, conjugated with two fusion proteins, ML0304 and ML0331, forming LID; NTO: natural trisaccharide-octyl; NT-P-BSA: natural trisaccharide phenylpropionyl bovine serum albumin; OD: optical density; PGL-I: phenolic glycolipid-I; ref. no.: reference number; UCP-LFA: up-converting phosphor lateral flow assay. (XLSX)