Isolation of Bacterial Cellulose Nanofibers Producing Acetic Acid Bacteria from Fermented juices

Bacterial cellulose (BC) are high purity, ultra-fine network of nanofibrils structure, and non-toxic. The nanofibrils bacterial cellulose used for many applications in the food, medical and cosmetics industries. The objectives of this study aim to isolate and identify of nanofibrils bacterial cellulose producing acetic acid bacteria (AAB). We studied, isolation of cellulose producing AAB from 26 samples of Kombucha culture and different kinds of fermented fruit, and herb juices on Histrin Schramm. One hundred and seventeen bacterial isolates were obtained. The isolates were tested for their biochemical characteristics and bacteria cellulose nanofibrils production. Four isolates: RC1, RC3, RJ1, and RG2 were found to produce highest of bacteria cellulose (approximately 9-11 mm) from 1% (v/v) concentration of acetic acid in HS broth. The morphology and nanofibers were investigated by SEM and XRD, the bacterial cellulose nanofibrils size was 4.10, 4.95, 4.38, and 4.91 nm, respectively. However, isolates RJ1, RC3, and RC1 were identified to Komagataeibacter hansenii and then, isolates RG2 was identified to K. maltaceti.