Analysis of transcriptional fidelity during heat stress in A. thaliana

Our goal is to describe the regulation and role of RNAPII transcriptional fidelity during heat stress in plants. For this we use circle-sequencing (CirSeq) on wild type Col-0, transcriptional (TFIIS) and post-transcriptional (UPF1) quality control mutant Arabidopsis thaliana seedlings treated with ambient (NT) and one day 37 C heat (1d) temperature. Our results have relevance in plant biology, molecular biology, abiotic stress biology an agriculture.