Transcription profiling of mouse neocortex to investigate age-related gene expression changes

Aging is associated with major nuclear changes affecting genomic integrity and gene expression. Here we compare the gene expression profiles in the neocortex of young (5 months old) and old (30 months old) B6xC3 F1 mice. In a related study, we compare genes that change expression with age to SIRT1 associated genes identified by ChIP on chip (NimbleGen) and find a significant overlap, indicating that SIRT1 target genes are preferentially deregulated with age. Experiment Overall Design: Total neocortex RNA was hybridized on Affymetrix M430 2.0 arrays. RNA was isolated from five young (5 months) and 5 old (30 months) B6xC3 F1 mice, samples were hybridized individually.衰老与诸多影响基因组完整性及基因表达的关键细胞核变化存在紧密关联。本研究对年轻（5月龄）与年老（30月龄）B6xC3 F1小鼠新皮层内的基因表达谱进行了对比分析。在一项相关研究中，我们将随衰老发生表达变化的基因，与通过染色质免疫沉淀芯片（ChIP-on-chip，NimbleGen平台）鉴定得到的沉默信息调节因子1（SIRT1）关联基因进行比对，发现二者存在显著的基因重叠，这表明SIRT1靶基因在衰老进程中更易出现表达失调。实验整体设计：提取全部新皮层总RNA，将其与Affymetrix M430 2.0基因表达芯片进行杂交。实验材料取自5只年轻（5月龄）及5只年老（30月龄）B6xC3 F1小鼠，所有样本均单独完成杂交反应。