Quantitative Analysis of mid-L4 WT worms after P. aeruginosa PA01 infection for 12 hours

To identify candidate genes whose expressions were regulated by P. aeruginosa PA01, we used RNA-Seq analysis to study the transcriptomic profiles of mid-L4 worms exposed to P. aeruginosa PA01 for 12 hours. Differential expression analysis was performed by comparing WT worms exposed to P. aeruginosa PA01 for 12 hours versus worms fed E. coli OP50. Overall design: The transcriptional profiles of mid-L4 WT worms exposed to P. aeruginosa PA01 or E. coli OP50 for 12 hours were generated by deep sequence, in quadruplicate, using Illumina Novaseq™.