Number of transformants after WGT of strain DE-3 and selection for improved HMF tolerance.
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Number of colonies on HMF-containing medium for each transformation condition with or without further subculturing of the colonies is indicated. All YPDX plates contained 6.5% D-glucose and 4.0% D-xylose. All visible colonies on YPDX 7.0 g/L HMF were replated on solid nutrient medium. The transformants that showed growth after replating, were subsequently subcultured in liquid YPDX without HMF for 10 serial transfers and then replated on YPDX with 7.0 g/L HMF.
创建时间:
2021-10-08



