Mass spectrometry analysis of Flag-H9N2-NS2 interacting candidates
收藏科学数据银行2024-06-18 更新2026-04-23 收录
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资源简介：
To identify SUMOylated factors that interact with NS2 via SIM-SUMO interaction, FLAG-tagged H9N2-NS2 was co-transfected into HEK293T cells together with plasmids encoding His-SUMO1 and Myc-Ubc9. After transfection for 24 hours, cells were infected with H9N2 AIV for a further 24 hours and Flag-NS2 was purified using anti-Flag beads. Purified protein complexes were analyzed by liquid chromatography tandem mass spectrometry (LC-MS/MS).
提供机构：
Huiling Ren; Xiaojun Wang; Mengmeng Yu; State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150069, China; Liuke Sun
创建时间：
2024-06-14
