Putative allergens identified in PepMV infected and non-infected tomato fruits.

Protein extracts from tomato fruits were separated by 2DE gel electrophoresis and analysed by immunoblotting with an IgE serum pool from nine tomato allergic subjects. Spots showing a reaction were eluted from parallel gels and identified using mass spectrometry. Mascot searches were done in 08/2011. Spots listed by numbers as identified in Figure 2. Abbreviations: −: −PepMV; (+): +PepMV; n.d.: not detectable with mass spectrometry; Lyc e 1 and Lyc e 2: confirmed tomato allergens by the International Union of Immunological Societies (IUIS).