Experimental adaptation to singular pathogen challenge reduces susceptibility to novel pathogens in Drosophila melanogaster

In the wild, hosts often encounter and must respond to novel pathogens, that is pathogens that they have not encountered in recent evolutionary history, and therefore are not adapted to. How hosts respond to these novel pathogens and the outcome of such infections can be shaped by the host’s evolutionary history, especially by how well adapted the host is to its native pathogens, that is pathogens they have evolved with. Host adaptation to one pathogen can either increase its susceptibility to a novel pathogen, due to specialization of immune defenses and trade-offs between different arms of the immune system or can decrease susceptibility to novel pathogens by virtue of cross-resistance. Using laboratory Drosophila melanogaster populations, we explore if hosts experimentally adapted to survive infection challenges by a particular bacterial pathogen are also better at surviving infection challenges by novel bacterial pathogens. We found that such hosts can survive infection challenges b..., A. Details of fly populations
 
EPN selection regime: The EPN selection regime consists of three populations (E, P, and N), each having four blocks (1-4). E populations are subjected to infection with bacteria Enterococcus faecalis every generation; P populations are sham-infected controls; N populations are uninfected controls. Populations within each block (viz. E1, P1, and N1) share a common ancestor. Blocks serve as both evolutionary and experimental/statistical replicates.
# IUS selection regime: The IUS selection regime consists of three populations (I, S, and U), each having four blocks (1-4). I populations are subjected to infection with bacteria Pseudomonas entomophila every generation; S populations are sham-infected controls; U populations are uninfected controls. Populations within each block (viz. I1, S1, and U1) share a common ancestor. Blocks serve as both evolutionary and experimental/statistical replicates.
 
B. Response to selection experiment
 
EPN selection regime: F..., , # Experimental adaptation to singular pathogen challenge reduces susceptibility to novel pathogens in Drosophila melanogaster.

[https://doi.org/10.5061/dryad.hdr7sqvtv](https://doi.org/10.5061/dryad.hdr7sqvtv)

## Description of the data and file structure

This file (\"Selection_line_cross_resistance_experiment.xlsx\") was generated in 2018-19 by Aparajita Singh and others at the Evolutionary Biology Lab, IISER Mohali.

GENERAL INFORMATION

1\. Title of Dataset: \"Recurrent evolution of cross-resistance in response to selection for improved post-infection survival in Drosophila melanogaster\"

2\. Author Information

A. Principal Investigator Contact Information

Name: Prof. N. G. Prasad

Institution: Indian Institute of Science Education and Research, Mohali

Address: IISER Mohali, Sector 81, Knowledge City, SAS Nagar, Punjab - 140306, India.

Email: [[e-mail]](mailto:[e-mail])

B. Associate or Co-investigator Contact Information

Name: Aparajita Singh

In...