Expression data from advanced non-small cell lung cancer

We used microarrays to detail the differentially expressed miRNA before and after down-regulation of miR-744 in lung cancer cell lines by genome-wide expression microarray To gain insights into the molecular mechanism of miR-744 in the promotion of malignant phenotype of lung cancer cells, we analyzed the mRNAs differentially expressed before and after miR-744 down-regulation. Lung cancer cells were transfected with antagomir-744 (IN1, IN2, IN3) or antagomir-NC(NC1, NC2, NC3) for RNA extraction and hybridization on Affymetrix microarrays by using genome-wide expression profile chip.