Effect of maternal iron deficiency on placental gene expression on gestational day 18.5 in rats

Iron deficiency occurs when iron demands chronically exceed intake, and is particularly prevalent in pregnant women. Iron deficiency during pregnancy poses health risks for the baby. The placenta serves as the interface between a pregnant mother and her baby; thus, maternal iron deficiency may indirectly impact fetal growth and development by altering placental function. In this study, pregnant Sprague-Dawley rats were fed either a low-iron or iron-replete diet starting two weeks before mating. On gestational day 18.5, RNA was collected, and a Clariom S microarray was performed to elucidate differences in gene expression between gestaional day 18.5 placentas isolated from dams fed iron replete or iron deficient diets.当机体铁需求长期超过摄入水平时，便会引发铁缺乏症，该病症在孕妇群体中尤为高发。孕期铁缺乏会对胎儿健康造成危害。胎盘作为孕母与胎儿之间的物质交换界面，母体铁缺乏可通过改变胎盘功能，间接影响胎儿的生长发育。本研究中，妊娠斯普拉格-道利（Sprague-Dawley）大鼠于交配前两周开始，分别被饲喂低铁日粮与铁充足日粮（iron-replete diet）。在妊娠第18.5天时，研究人员采集核糖核酸（RNA）样本并开展Clariom S微阵列（Clariom S microarray）检测，以阐明从饲喂铁充足日粮或铁缺乏日粮的妊娠母鼠体内分离得到的妊娠第18.5天胎盘组织的基因表达差异。