Effects of the dietary inclusion of H. illucens larvae meal as fishmeal replacer on intestinal microbial community of rainbow trout

A 12-week feeding trial was conducted on rainbow trout (Onchorhynchus mykiss).Two diets were formulated to be isonitrogenous, isolipidic and isoenergetic (mancano CP, EE,GE). The control diet (Ctrl) contained only fishmeal (FM) as animal protein source, while the experimental diet (Hi15) differed by Ctrl because FM was totally substituted by defatted Hermetia illucens (Hi) larvae meal, corresponding to dietary inclusion level of Hi meal equal to 15%.At the end of the feeding trial eight fish/dietary group were sampled and intestine was aseptically isolated from each fish. The faecal matter was obtained by squeezing out and scrapping the intestinal mucosa with a sterile spatula, in order to collect both, the digesta- and the mucosa associated microbiota (transit- and resident microbiota.