CHIP-seq in THP1 cells

In our model, p53 is acting through recruitment of SIRT1 to reduce histone acetylation. A common myeloid cell line THP-1 are p53 mutant cells that have a significant deletion in p53 coding sequence leading to loss of p53 expression.We immunoprecipitated chromatin in THP1 cells using antibodies against H3K27ac and SIRT1 to identify the bingding peak in NEAT1 gene promoter to confirm P53' function Overall design: We immunoprecipitated chromatin in THP1 cells using antibodies against H3K27ac and SIRT1 to identify the potential binding genes.