Detection on the effect of TLR stimulation and NOS inhibition on human chondrocytes.. Detection on the effect of TLR stimulation and NOS inhibition on human chondrocytes.

To enlarge our understanding on the effect of TLR1/2 stimulation to human chondrocytes and how NOS inhibition blocks this stimulatory effects, we planned to apply RNAseq analysis to human chondrocytes that are treated with TLR1/2 agonists and NOS inhibitor. Overall design: During human chondrocyte sphroid culture, TLR1/2 agonist PamCSK4 and NOS inhibitor L-NAME were added. After four days of culture under hypoxia condition, RNA were isolated from the spheroids and subjected to RNAseq analysis.