Single-cell transcriptomic analysis Treg cells in peripheral lymph nodes

Treg cell-specific ablation of Cxxc1 leads to the development of a lethal inflammatory disease characterized by spontaneous T-cell activation.Cxxc1-deficient Treg cells showed reduced immunosuppressive capacity without affecting Treg cell numbers.To investigate the role of Cxxc1 as a transcriptional regulator of Treg subsets, we performed single-cell RNA-seq (scRNA-seq) paired with T cell receptor sequencing (TCR-seq) . CD4+YFP+ Treg cells from WT and Foxp3Cre Cxxc1fl/fl mice were isolated for scRNA-seq.