Customizing CRISPR-Cas PAM specificity with protein language models
收藏NIAID Data Ecosystem2026-05-10 收录
下载链接：
https://www.ncbi.nlm.nih.gov/sra/SRP656075
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资源简介：
We developed Protein2PAM, an evolution-informed protein language model that predicts PAM specificity of CRISPR-Cas proteins. Using in silico mutagenesis and model-guided evolution, we generated Cas9 variants with broadened PAM compatibility and increased activity, enabling editing of genomic sites that were previously inaccessibile due to PAM constraints.
创建时间：
2025-12-20
