Gene expression profile at single cell level of CD4+ T cells from the lymph nodes of young and old DBA/1 mice with or without glucose-6-phosphate isomerase (GPI)-induced arthritis (GIA)

It has been suggested that CD4+ T cell senescence is involved in the pathogenesis of elderly-onset rheumatoid arthritis (EORA). We performed scRNA-seq analysis using CD4+ T cells collected from inguinal lymph nodes of young and old DBA/1 mice to investigate the association between aging CD4+ T cells and elderly-onset arthritis. Inguinal lymph nodes (LNs) were harvested from young (8 weeks) and old (14-15 months) DBA/1 mice 14 days after GIA induction (GIA groups) or PBS injection (control groups). CD4+ T cells were isolated by cell sorting system equally from four mice in each of the four groups, each with 10,000 cells, and analyzed using scRNAseq.