Assessment of DNA extraction methods for human gut mycobiome analysis using mock community

The gut mycobiome plays an important role in the health and disease of the human gut, but its exact function is still under investigation. While there is a wealth of information available on the bacterial community of the human gut microbiome, research on the fungal community is still relatively limited. In particular, technical methodologies for mycobiome analysis, especially the DNA extraction method for human fecal samples, varied in different studies. A mock fungal community with a known composition of fungal strains was also generated and included to compare different DNA extraction methods.