<i>De novo</i> transcriptome sequencing of genome analysis provides insights into <i>Solidago canadensis</i> invasive capability via photosynthesis

<i>Solidago canadensis</i> is one of the most destructive invasive plants in the East of China, yet nothing is known about its photosynthetic ability at the molecular level. In order to examine the mechanism aiding invasion from the photosynthetic and molecular perspective, transcriptome sequencing and <i>de novo</i> assembly of <i>S. canadensis</i> and its congener <i>S. decurrens</i> (native, non-invasive species) were compared in a bioassay experiment. Results showed that <i>S. canadensis</i> has higher biomass and net photosynthetic rates than those of the native plant, <i>S. decurrens</i> (<i>P </i>&lt; 0.05). Based on RNA-seq data, the genes which are closely related to light absorption and electron transfer in <i>S. canadensis</i> were observed to be up-regulated compared with those from <i>S. decurrens</i>, including the process of photosystems I and II, such as <i>PsbP</i>, <i>PsbQ</i>, <i>Psb27</i>, <i>PsbW</i>, <i>PsaG</i>, <i>PetE</i>, and light-harvesting complexes (LHCs). The RT-qPCR verification results of key genes were similar to those from transcriptome. Therefore, our results might partly explain the successful invasion of <i>S. canadensis</i> in China at the level of transcriptome, leading to its enhanced photosynthetic capability.

加拿大一枝黄花（Solidago canadensis）是中国东部地区危害最为严重的外来入侵植物之一，但目前学界对其光合能力的分子机制仍知之甚少。为从光合与分子层面探究其助入侵的内在机制，本研究通过生物测定实验，对加拿大一枝黄花及其同属非入侵本土近缘物种翅茎一枝黄花（S. decurrens）开展转录组测序与从头（de novo）组装分析并进行比较。结果显示，相较于本土非入侵植物翅茎一枝黄花，加拿大一枝黄花具有更高的生物量与净光合速率（P < 0.05）。基于转录组测序数据，加拿大一枝黄花中与光吸收、电子传递紧密相关的基因表达量显著上调，涵盖光系统I、光系统II相关通路，包括PsbP、PsbQ、Psb27、PsbW、PsaG、PetE以及光捕获复合体（LHCs）。关键基因的逆转录实时定量PCR（RT-qPCR）验证结果与转录组分析结果高度一致。因此，本研究结果从转录组层面部分解释了加拿大一枝黄花在中国成功入侵的机制，即其光合能力得到显著增强。

搜集汇总

背景与挑战

背景概述

该数据集通过比较入侵植物加拿大一枝黄花和本地一枝黄花的转录组测序数据，揭示了入侵物种在光合作用相关基因（如光系统I和II组分）上的上调表达，从而从分子层面解释其更高的光合能力和入侵成功。数据集包括RNA-seq原始数据和RT-qPCR验证结果，支持植物入侵机制的光合作用假说。