TcCPx and TcMPx expression in pROCK or MutT parasites.

T. cruzi epimastigotes lysates were prepared with exponential phase cultures after 20 min incubation or not with 50 µM H2O2. Protein extracts were quantified by Bradford assay and resolved by SDS-PAGE (A) (30 µg protein/lane). Western blot analysis of TcCPx and TcMPx from non-treated pROCK (1) and MutT (2), or 50 µM H2O2-treated pROCK (3) and MutT (4) parasites. β-tubulin was used as loading control. (B) Coomassie blue staining of the protein extracts. The best representative of three independent experiments is shown. The signal intensity obtained for each studied enzyme from pROCK untreated control was set to 100%, and the enzyme levels from the others parasites were evaluated as a percentage of the control. The results were expressed in graphs for each enzyme: TcCPx (C) and TcMPx. (D) The asterisk symbol (*) refers to significant differences from the pROCK non-treated control (*** P<0.001, one-way ANOVA test with Bonferroni post-test).