JBC 2024 - LECT2 Flow Shear

These Figshare files consist of raw/unprocessed images in various formats, processed data and statistical analysis (Graphpad Prism), high resolution versions of some images in the paper, and MATLAB scripts used to analyze/process the microfluidic data.Figure2.zip-Raw light microcsope images of microfluidic aggregates in Zeiss .czi formatFigure3.zip-GraphPad Prism files containing processed data derived from images in Figure 2, and statistical analysisFigure4.zip-Raw light microscope images of microfluidic aggregates from seeding experiments, in Zeiss .czi formatFigure6.zip-Raw EM images of negatively-stained samples in Gatan .dm3 format-Raw cryo-EM image of frozen vitrified sample in .mrc format-2D average of fibrils-Power spectrum calculated from 2D averageFigure7.zip-GraphPad Prism file containing processed data derived from images in Figure 4, and statistical analysisFigureS3.zip-Raw light microscope images of microfluidic aggregates in Zeiss .czi format, from another biological repeatFiguresS5-S11.zip-Raw light microscope images of microfluidic aggregates in Zeiss .czi format-GraphPad Prism files containing processed data derived from above images, and statistical analysisFigureS12.zip-Raw light microscope images of microfluidic aggregates in Zeiss .czi format, testing dependence of aggregation on protein concentration-Graphpad Prism file containing processed data derived from above images, and statistical analysisFigureS14.zip:-Raw light microscope images of microfluidic aggregates from additional biological repeats of seeding experiments, in Zeiss .czi format-GraphPad Prism files containing processed data derived from above images, and statistical analysisProcessChannelsV6.zip-MATLAB scripts for calculating areas and intensities of aggregates in microfluidic channels