Single-cell and spatial transcriptomics analysis of tobacco-associated lung adenocarcinoma development, and in response to targeting inflammation alone or in combination with immune checkpoint blockade in precancerous phase [Spatial transcriptomics]

Understanding cellular processes underlying early lung adenocarcinoma (LUAD) development is needed to devise intervention strategies. Here, we performed single-cell RNA sequencing (scRNA-seq) of mouse lungs from Gprc5a-/- mice during lung tumor development and also following therapeutic targeting of inflammation (IL-1??) alone and in combination with immune checkpoint blockade in precancerous stages. We coupled scRNA-seq analysis with spatial transcriptomics of mouse lungs under different conditions. Overall design: Gene expression profiling by high throughput single-cell sequencing and spatial transcriptomics