Gene expression profiling in the puf4? (midlog), and gcn4? (midlog and oxidative stress) mutants of Cryptococcus neoformans.

This study aimed to investigate transcriptomic changes in Cryptococcus neoformans mutants. We report transcriptome wide changes in the puf4? under basal growth conditions (30°C, midlog, YPD). We report transcriptome remodeling following hydrogen peroxide (H2O2) treatment in wildtype (H99) and gcn4? cells. Overall design: Cells were grown to midlog growth stage at 30°C, then indicated samples were treated with 1 mM H2O2 for 1 hour. Cells were pelleted and total RNA were extracted, DNase treated and sequenced using Illumina HiSeq platform.