Combining TSS-MPRA and sensitive TSS profile dissimilarity scoring to study the sequence determinants of transcription initiation

Using a transcription start site-capturing massively parallel reporter assay (TSS-MPRA) that simultaneously measures the location and frequency of transcription initiation from synthetic sequences, we carefully characterize the degree to which plasmid-based MPRAs recapitulate endogenous initiation patterns. TSS-MPRA sequencing of insert libraries to characterize transcription initiation patterns (K562)