Odor-induced phosphorylation of olfactory cilia proteins.
收藏PubMed Central1992-12-15 更新2026-05-16 收录
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https://pmc.ncbi.nlm.nih.gov/articles/PMC50682/
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资源简介：
Stimulation of isolated rat olfactory cilia in the presence of [gamma-32P]ATP leads to a significantly enhanced incorporation of [32P]phosphate. Depending on the type of odorants applied, the induced phosphorylation is completely blocked by specific inhibitors of either protein kinase A or protein kinase C. Time-course experiments indicate that the odor-induced modification of ciliary proteins is transient; the intensity of labeling decayed over time (1-10 sec). Separation of ciliary proteins by SDS/polyacrylamide gel electrophoresis followed by autoradiography demonstrated that upon stimulation with lilial, a single polypeptide (50,000 Da) was phosphorylated; the size of the modified protein is in line with the hypothesis that odorant receptors are phosphorylated subsequent to activation by specific odors. IMAGES:
提供机构：
National Academy of Sciences
创建时间：
1992-12-15
