Supplemental Material for Edskes, et al., 2021

The
Supplementary Information includes extended SILAC methods, strains of S. cerevisiae (Table S1), primers used
(Table S2), proteins increased 1.5-fold or more in abundance in pre9Δ (Table S3), Proteins decreased 1.5-fold or more
in abundance in pre9Δ (Table S4), a
diagram of the proteasome α ring (Fig. S1), evidence that proteasome – related
knockouts do not affect [PSI+] propagation (Fig. S2), evidence that [URE3-1] is
less stable in 779-6A pre9Δ than in
BY241 pre9Δ (Fig. S3), data showing
that levels of Sup35p are not affected by proteasome – related mutations in
strain BY241 (Fig. S4), data showing Btn2p levels in proteasome mutants are
elevated by the presence of [PSI+] (Fig. S5 and S6), measuring the absolute levels of Btn2p and
Cur1p in strain 779-6A by comparison with purified recombinant Btn2-His6 and
Cur1-His6 (Fig. S7), the multiple bands of Cur1p (Fig. S8), the elevation of
Btn2p in tof2 mutants (Fig. S9) and
the absence of a ubiquitin ladder of Btn2p in a pre9Δ strain (Fig. S10).
Table S5 compares protein turnover rates with their levels by SILAC in pre9Δ strains.