miRNA profiling in DVT patients and control subjects

Microarray and quantitative real-time PCR (qRT-PCR) results showed that the Caspase-1 expression was increased while miR-513c-5p level was decreased substantially in DVT patients, and there was significant negative correlation between miR-513c-5p and Caspase-1. Knockdown of miR-513c-5p could aggravate DVT formation by enhancing Caspase-1-mediated pyroptosis, while overexpression of miR-513c-5p could alleviate DVT formation via inhibiting Caspase-1 expression. we identified pyroptosis of PBMCs with elevated expression of Caspase-1 in DVT patients. miRNA microarray analysis revealed miR-513c-5p as the most significantly down-regulated miRNA in DVT and the receiver operating characteristic (ROC) analysis showed that miR-513c-5p level could sensitively discriminate DVT from healthy controls. Total RNA was extracted from PBMCs using TRIzol (Invitrogen, Carlsbad, CA, USA). The genome-wide analysis of miRNA expression in DVT patients (n= 6) and control subjects (n= 6) were performed using Human miRNA, Release 21.0 (8*60K, Design ID:070156).