Genes specific to cowpea: cowpea vs adzuki bean

Comparative hybridization analysis Microarray-based genomic hybridization was used here as a high-throughput analog to traditional southern hybridization, which is the classical standard method for detecting specific DNA fragments in a genome. Genomic DNA from cowpea and adzuki bean were labeled with fluorochromes Cy3 and Cy5, respectively, purified, combined, and mixed with SeqCap EZ Developer Reagent to hybridize with the Agilent SurePrint G3 Custom Microarray representing 29,728 unigenes from the cowpea unigene assembly P12.