scRNA-Seq analysis of lineage negative compartments of miR-221/222-proficient and deficient mice.

To study the effect of miR-221/222-deficiency on early hematopoietic lineage differentiation pathways on sorted multipotent progenitor (MPP), common lymphoid progenitor (CLP), lineage-ckit+Sca1- and lineage-ckit-Sca1- populations of miRNA-proficient and deficient mice. Comparative gene expression profiling analysis on MPP, CLP, lineage-ckit+Sca1- and lineage-ckit-Sca1- cell populations of miR-221/222-proficient and deficient mice using single-cell RNA-Seq.