Single-cell RNA Seq of hematopoietic stem and progenitor cells

This dataset is part of a study that investigated how the hematopoietic system coordinates the rapid and efficient regeneration of the megakaryocytic lineage during stress scenarios. We found that the phenotypic hematopoietic stem cell (HSC) compartment contains stem-like megakaryocyte-committed progenitors (SL-MkPs), a cell population that shares many features with multipotent HSCs and serves as a lineage-restricted emergency pool for inflammatory insults. This dataset contains single-cell RNA sequencing data of 30 hematopoietic stem and progenitor cells which, in the context of our study, confirmed that MK-specfic transcripts are of highly variable expression in HSCs. The dataset further showed that variations in MK transcript expression in HSCs is not correlated with global transcriptomic rearrangements. Murine bone marrow cells were sorted by Lin-cKit+CD150+CD48- (referred to as cd150+ in the following) and Lin-cKit+CD150- (referred to as cd150- in the following). Transcriptomes of 11 cd150- and 9 cd150+ HSCs were determined using QUARTZ, a single-cell RNASeq protocol本数据集隶属于一项探究造血系统如何在应激状态下协调巨核细胞系快速高效再生的研究。本研究发现，表型造血干细胞（hematopoietic stem cell, HSC）群体中存在巨核细胞定向干样祖细胞（stem-like megakaryocyte-committed progenitors, SL-MkPs）：这类细胞群体与多能造血干细胞共享诸多特征，并可作为应对炎性损伤的谱系限制性应急储备库。本数据集包含30个造血干祖细胞的单细胞RNA测序数据，在本研究语境下，该数据证实造血干细胞内巨核细胞特异性转录本的表达存在高度异质性。该数据集还进一步揭示，造血干细胞内巨核细胞转录本的表达变异与全局转录组重排并无关联。研究人员通过Lin⁻cKit⁺CD150⁺CD48⁻（下文简称cd150⁺）与Lin⁻cKit⁺CD150⁻（下文简称cd150⁻）对小鼠骨髓细胞进行分选。本研究采用单细胞RNA测序方案QUARTZ，完成了11个cd150⁻与9个cd150⁺造血干细胞的转录组检测。