The role of the DRW1 overexpression on the gene expression levels and RNA m5C levels in rice grains.

DRW1 controls gene expression contributed to hormone pathway, photosynthesis, carbon metabolism as well as cell division and proliferation . DRW1 controls RNA m5C levels of transcripts contributed to hormone pathway, photosynthesis, carbon metabolism. Samples were collected from ZH11 and DRW1-OE grains at 3 days after pollination. Total RNA was extracted using a Plant RNA Kit (RP3302; Bioteke, Beijing, China), and high-throughput RNA-sequencing (RNA-seq) was conducted using the Illumina nova6000 platform. Samples were collected from ZH11 and DRW1-OE1 seedlings at 3 weeks after germination.~300 μg total RNA was fragmentated by ZnCl2, and RNA fragments are used for m5C immunoprecipitation. High-throughput RNA-sequencing (RNA m5C IP-seq) was conducted using the Illumina nova6000 platform.