Analysis of microRNAs from osteoblast and osteoclast derived small extracellular vesicles using high through-put miRNA sequencing. Analysis of microRNAs from osteoblast and osteoclast derived small extracellular vesicles using high through-put miRNA sequencing

To gain insight into the microRNA expression profile of small extracellular vesicles derived from bone metabolism related cell types and to verify their mechanism, we utilized the miRNA sequencing technology to analyze the miRNA profiles of different mouse osteoblast and osteoclast cell derived small extracellular vesicles. Overall design: We collect the small extracellular vesicles from the supernatant of osteoclast and osteoblst cells using ultracentrifugation method. The small extracellular vesicles were then extracted for small RNAs to perform high through-put sequencing