Macrophage responses to zinc-limited and zinc-replete Mycobacterium tuberculosis H37Rv

The goal of this experiment was to determine if macrophage responses differ between macrophages infected with M. tuberculosis H37Rv grown in the standard, zinc-replete medium compared to cells grown in zinc-limited medium. The macrophage responses after 4 hours and 24 hours of infection were determined by isolating the RNA and analyzed it with the NanoString panel mouse host response panel. There were 15 samples total: RAW 264.7 macrophages infected with zinc-limited M. tuberculosis after 4 hours of infection, RAW 264.7 macrophages infected with zinc-replete M. tuberculosis after 4 hours of infection, uninfected RAW 264.7 macrophages after 24 hours, RAW 264.7 macrophages infected with zinc-limited M. tuberculosis after 24 hours of infection, RAW 264.7 macrophages infected with zinc-replete M. tuberculosis after 24 hours of infection, all in biological triplicate.