Effect of 10 nights of hypoxia on glucose homeostasis, appetite, gut microbiota and inflammation in adults with type 2 diabetes: A single-blind, randomised sham-controlled, crossover trial

This study aims to examine the effect of repeated overnight hypoxic exposure on blood glucose control in adults with type 2 diabetes mellitus (T2DM) and provide insight into the mechanisms underpinning any beneficial effects. Thirteen adults with T2DM completed a single-blind, randomised, balanced, sham-controlled, crossover study. The gut microbiota were assessed before and after 10 nights of sleeping in 'hypoxic tents' (FIO2=0.155) and a normoxic sham (FIO2=0.209), using full-length 16S rRNA amplicon sequencing, based on Oxford Nanopore Technologies (ONT) nanopore sequencing. Repeated overnight moderate hypoxia did not significantly affect affect microbial diversity and abundance.