Alterations in serum microRNA in humans with alcohol use disorders impact cell proliferation and cell death pathways and predict structural and functional changes in brain [rat miRNA-Seq]

We performed a global analysis of extracellular miRNAs in the serum of human subjects diagnosed with Alcohol Use Disorder (AUD) to identify robust biomarkers of early brain damage or dysfunction. This was performed in a set of 20 AUD subjects and 10 age-matched controls. They were subjected to comprehensive medical, neuropsychological and neuroimaging tests, followed by comparison of miRNA levels found in peripheral blood serum. The levels of miRNAs were quantified using two independent high-throughput methods: Affymetrix microarray and Illumina next-generation RNA-sequencing. Cross-species validation was performed using rat drinking models and mouse neural stem cell culture models, with tissue specificity of the serum miRNAs examined using additional RNA-sequencing of serum and body tissues in untreated rats. This data series includes the rat brain and body tissue miRNA data obtained from RNA-sequencing only and is part of a larger SuperSeries (GSE71579) now being curated at GEO本研究对确诊为酒精使用障碍（Alcohol Use Disorder, AUD）的人类受试者血清中的细胞外微小RNA（microRNA, miRNA）开展全谱分析，以筛选稳健的早期脑损伤或功能障碍生物标志物。本研究纳入20名AUD患者与10名年龄匹配的健康对照者，对所有受试者开展全面的医学评估、神经心理学测试及神经影像学检查，随后对比其外周血清中的miRNA水平。miRNA水平通过两种独立的高通量方法完成定量：Affymetrix微阵列（Affymetrix microarray）与Illumina下一代RNA测序（Illumina next-generation RNA-sequencing）。跨物种验证采用大鼠饮酒模型与小鼠神经干细胞培养模型开展，同时通过对未处理大鼠的血清及全身组织进行额外RNA测序，以检测血清miRNA的组织特异性。本数据集仅包含通过RNA测序获得的大鼠脑组织与全身组织miRNA数据，属于更大规模的超级数据集（SuperSeries，GSE71579）的一部分，该超级数据集目前正在基因表达综合数据库（Gene Expression Omnibus, GEO）中进行编目。