Gene expression of p53 null vs. p53 wild-type mouse kidneys at embryonic day 15.5

Gene expression microarray analysis was performed on E15.5 p53+/+ and p53-/- litter-matched kidneys. We have previously shown that p53 is expressed in both UB and MM lineages in the kidney, and that p53-null embryos on C57B6L background exhibit a range of congenital abnormalities of the kidney and urinary tract such as duplicated ureters, reduced nephron numbers, and compromised nephron progenitor renewal and differentiation. Here our goal was to reveal the p53 transcriptome in mouse embryonic kidneys at E15.5. This is a two-condition experiment comparing gene expression between p53 null vs. p53 wild-type mouse kidneys at embyronic day 15.5. Three biological replicates (rep1-3) and their dye-swapped experimental replicates are included, in which rep1 has a duplicate and a duplicate dye-swap experiments.本研究对胚胎发育第15.5天（E15.5）的p53纯合野生型（p53+/+）与p53纯合敲除（p53-/-）同窝配对小鼠肾脏开展了基因表达微阵列分析。此前我们已证实，p53在小鼠肾脏的输尿管芽（Ureteric Bud, UB）与后肾间充质（Metanephric Mesenchyme, MM）谱系中均有表达；且在C57B6L遗传背景下，p53敲除胚胎会出现一系列肾脏与泌尿系统先天性畸形，包括输尿管重复、肾单位数量减少，以及肾单位祖细胞更新与分化功能受损。本研究的核心目标为解析胚胎发育第15.5天小鼠胚胎肾脏中的p53转录组。本实验为双条件对照研究，旨在比较胚胎发育第15.5天p53敲除与野生型小鼠肾脏的基因表达差异。本研究包含3组生物学重复（rep1-3）及其对应的染料互换实验重复；其中rep1设有1组平行重复与1组染料互换平行重复实验。