Lactobacillus kunkeei alleviated acetamiprid-induced symbiotic microbiota dysregulation in honeybee

Nowadays, Colony Collapse Disorder extensive struck honeybees and leading to the disappearance and death of number of honeybees. The insecticides were considered to critical factor, included acetamiprid. Lactic acid bacteria (LAB) as probiotics to improve health of human and animal or against pathogens was widespread administered. We speculated supplement with LAB could alleviated acetamiprid-induced health injury in honeybee. Lactobacillus kunkeei was isolated from beebread. It was treated with honeybees alone or in combination with acetamiprid. Based on 16S rRNA pyrosequencing, information of intestinal bacterium on honeybees were acquired. The results showed that supplement with L. kunkeei significantly increased the survival of honeybee and decreased pollen consumption by honeybee under Acetamiprid exported. Under acetamiprid exported, some opportunistic bacteria and pathogenic invaded intestinal of honeybee. The community richness and community diversity of symbiotic microbiota were decreased. The community structure of intestinal bacterium was changed and differenced. However, supplement with L. kunkeei, the community richness and community diversity of symbiotic microbiota was observed with upward trend and the community structure was stabilized. The abundance of core bacteria (Bifidobacterium sp. and Snodgrassella alvi) was increased. This research demonstrated that supplement with LAB alleviated acetamiprid-induced health injury in honeybee and clarified the mechanism based on intestinal bacterium.